查看“︁酒石酸脱氢酶”︁的源代码

{{enzyme
| Name = 酒石酸脱氢酶
| Systematic_name = tartrate:NAD<sup>+</sup> oxidoreductase
| Acronym = TDH
| Other_names = mesotartrate dehydrogenase
| EC_number = 1.1.1.93
| CAS_number = 37250-29-6
| IUBMB_EC_number = 1/1/1/93
| GO_code = 0009027
| image = 3fmx.jpg
| width = 270
| caption = 
}}
'''酒石酸脱氢酶'''（{{lang-en|tartrate dehydrogenase}}，[[EC编号|EC]] [http://enzyme.expasy.org/EC/1.1.1.93 1.1.1.93] {{Wayback|url=http://enzyme.expasy.org/EC/1.1.1.93 |date=20201113143911 }}），是一种以[[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]]或[[烟酰胺腺嘌呤二核苷酸磷酸|NADP<sup>+</sup>]]为[[受体 (生物化学)|受体]]、作用于[[供体]][[次甲基|CH]]-[[羟基|OH]][[基团]]上的[[氧化还原酶]]。这种[[酶]]能[[催化]]以下[[酶促反应]]：

:[[酒石酸]] + [[烟酰胺腺嘌呤二核苷酸|NAD]]<sup>+</sup> + [[二氧化碳|CO<sub>2</sub>]]<math>\rightleftharpoons</math> [[草酰羟乙酸]] + NADH + [[氢离子|H<sup>+</sup>]]

酒石酸脱氢酶是一种具有[[立体特异性]]（stereospecific）的[[胞内酶]]（intracellular enzyme）。<ref>{{cite journal | author = R. Harve and R. K. Bajpai | title = Production and purification of tartrate dehydrogenase: Role of aqueous two-phase extraction | journal = Applied Biochemistry and Biotechnology | volume = 70-72 | pages = 677-686 | doi = 10.1007/BF02920179 | url = http://www.springerlink.com/content/71678n0t92765184/ | date = Nov 1, 1998 | access-date = 2012-08-01 | archive-url = https://web.archive.org/web/20190614071504/https://link.springer.com/article/10.1007%2FBF02920179 | archive-date = 2019-06-14 | dead-url = yes }}</ref>这种酶人们发现的第三个[[金属]][[离子]]依赖性、[[脱羧反应|脱羧型]]''R''-羟酸脱氢酶，可以将[[内消旋化合物|内消旋]]酒石酸和L-酒石酸作为[[底物]]，并需要[[二价锰离子|Mn<sup>2+</sup>]]和一价[[阳离子]]作为[[辅因子]]。可能是因为经历的[[自然选择]]的时间不长，酒石酸脱氢酶对酒石酸的催化能力较弱。[[恶臭假单胞菌]]（''Pseudomonas putida''）[[基因]]编码的酒石酸脱氢酶与[[原核生物]]的[[异丙基苹果酸脱氢酶]]具有很高的[[同源|同源性]]，但其酒石酸脱氢酶具有独特的NAD<sup>+</sup>结合域。酒石酸脱氢酶在以[[异丙基苹果酸]]为底物时[[酶促反应]]的[[米氏常数]]''K''<sub>m</sub>为14±2μM。<ref>{{cite journal | author = P.A. Tipton, B.S. Beecher | title = Tartrate Dehydrogenase, a New Member of the Family of Metal-Dependent Decarboxylating ''R''-Hydroxyacid Dehydrogenases | journal = Archives of Biochemistry and Biophysics | volume = 313 | issue = 1 | pages = 15-21 | doi = 10.1006/abbi.1994.1352 | url = http://www.sciencedirect.com/science/article/pii/S000398618471352X | date = Aug 1994 | access-date = 2012-08-01 | archive-date = 2019-06-14 | archive-url = https://web.archive.org/web/20190614071504/https://www.sciencedirect.com/science/article/abs/pii/S000398618471352X | dead-url = no }}</ref>

== 参考文献 ==
{{reflist}}
{{refbegin}}
* {{cite journal | author = Kohn LD, Packman PM, Allen RH, Jakoby WB | date = 1968 | title = Tartaric acid metabolism. V. Crystalline tartrate dehydrogenase | journal = J. Biol. Chem.  | volume = 243 | pages = 2479-2485  | pmid = 4297261 | issue = 10 }}
{{refend}}

{{EC 1.1}}

[[Category:EC 1.1.1]]
[[Category:NADH依赖性酶]]
[[Category:锰酶]]
[[Category:未知结构的酶]]