查看“︁脱乙酰氧基头孢菌素-C合酶”︁的源代码

{{enzyme
| Name = 脱乙酰氧基头孢菌素-C合酶
| EC_number = 1.14.20.1
| CAS_number = 85746-10-7
| IUBMB_EC_number = 1/14/20/1
| GO_code = 0050599
| image = 
| width = 
| caption = 
}}
'''脱乙酰氧基头孢菌素-C合酶'''（{{lang-en|Deacetoxycephalosporin-C synthase}}；{{EC number|1.14.20.1}}）在[[酶学]]中是一个[[催化]]如下[[化学反应]]的酶。

:青霉素N + α-酮戊二酸 + O<sub>2</sub> <math>\rightleftharpoons</math> 脱乙酰氧基头孢菌素C + 琥珀酸 + CO<sub>2</sub> + H<sub>2</sub>O

[[File:Deacetoxycephalosporin-C synthase.svg|600px|center]]

此酶的三种[[底物]]分别是[[青霉素N]]、[[α-酮戊二酸]]与[[氧气]]，而它的四种[[产物 (化学)|产物]]则分别是[[脱乙酰氧基头孢菌素C]]、[[琥珀酸]]、[[二氧化碳]]与[[水]]。

此酶属于[[氧化还原酶]]家族，特异性地作用于配对的供体，并以氧气作为氧化剂结合或还原氧气。被结合的氧无需借由α-酮戊二酸作为一个供体或是被脱氢的方式被传递。此酶类的系统命名为'''青霉素-N,2-酮戊二酸：氧氧化还原酶（扩环）'''。通常使用的其他名称包括了'''DAOCS'''、'''青霉素N扩环酶'''与'''DAOC合酶'''。此酶参与[[青霉素与头孢菌素生物合成]]。

==结构研究==
2007年7月下旬时此类酶的[[四级结构|结构]]已被解决，其[[蛋白质数据库]]新增编号为{{PDB link|1UNB}}、{{PDB link|1UO9}}、{{PDB link|1UOB}}、{{PDB link|1UOF}}、{{PDB link|1UOG}}、{{PDB link|1W28}}与{{PDB link|1W2A}}。

==参考文献==
{{reflist|1}}
* {{cite journal | author = Cantwell C, Beckmann R, Whiteman P, Queener SW, Abraham EP | date = 1992 | title = Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: construction of a new fungal biosynthetic pathway | journal = Proc. R. Soc. Lond. B. Biol. Sci.  | volume = 248 | pages = 283–9  | pmid = 1354366 | doi = 10.1098/rspb.1992.0073 | issue = 1323 }}
* {{cite journal | author = Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ | date = 2001 | title = Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS) | journal = J. Mol. Biol.  | volume = 308 | pages = 937–48  | pmid = 11352583 | doi = 10.1006/jmbi.2001.4649 | issue = 5 }}
* {{cite journal | author = Yeh WK, Ghag SK and Queener SW | date = 1992 | title = Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C Function, evolution, refolding, and enzyme engineering | journal = Ann. N.Y. Acad. Sci.  | volume = 672 | pages = 396–408 | doi = 10.1111/j.1749-6632.1992.tb32705.x }}
* {{cite journal | author = Andersson I | date = 1998 | title = Structure of a cephalosporin synthase | journal = Nature.  | volume = 394 | pages = 805–9  | pmid = 9723623 | doi = 10.1038/29575 | last2 = Van Scheltinga | first2 = AC | last3 = Lloyd | first3 = MD | last4 = Hara | first4 = T | last5 = Ramaswamy | first5 = S | last6 = Perrakis | first6 = A | last7 = Thompson | first7 = A | last8 = Lee | first8 = HJ | last9 = Baldwin | first9 = JE | issue = 6695 }}
* {{cite journal | author = Dotzlaf JE, Yeh WK | date = 1989 | title = Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus | url = https://archive.org/details/sim_journal-of-biological-chemistry_1989-06-15_264_17/page/n522 | journal = J. Biol. Chem.  | volume = 264 | pages = 10219–27  | pmid = 2656705 | issue = 17 }}
{{双加氧酶类}}
[[Category:EC 1.14.20]]
[[Category:已知结构的酶]]