查看“︁磷酸葡萄糖酸脱氢酶 (脱羧)”︁的源代码

{{enzyme
| Name = 磷酸葡萄糖酸脱氢酶 (脱羧)
| Systematic_name = 6-phospho-D-gluconate:NADP<sup>+</sup> 2-oxidoreductase (decarboxylating)
| Acronym = 
| Other_names = phosphogluconic acid dehydrogenase; 6-phosphogluconic dehydrogenase; 6-phosphogluconic carboxylase; 6-phosphogluconate dehydrogenase (decarboxylating); 6-phospho-D-gluconate dehydrogenase
| EC_number = 1.1.1.44
| CAS_number = 9073-95-4 | IUBMB_EC_number = 1/1/1/44 | GO_code = 0004616 
| image = 1pgq.jpg
| width = 270
| caption = 
}}
'''磷酸葡萄糖酸脱氢酶 (脱羧)'''（{{lang-en|phosphogluconate dehydrogenase (decarboxylating)}}，[[EC编号|EC]] [http://enzyme.expasy.org/EC/1.1.1.44 1.1.1.44] {{Wayback|url=http://enzyme.expasy.org/EC/1.1.1.44 |date=20120324084027 }}）是一种以[[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]]或[[烟酰胺腺嘌呤二核苷酸磷酸|NADP<sup>+</sup>]]为[[受体 (生物化学)|受体]]、作用于[[供体]][[次甲基|CH]]-[[羟基|OH]][[基团]]上的[[氧化还原酶]]。这种[[酶]]能[[催化]]以下[[酶促反应]]：

:6-[[磷酸]]-D-[[葡萄糖酸]] + [[烟酰胺腺嘌呤二核苷酸磷酸|NADP<sup>+</sup>]] <math>\rightleftharpoons</math> D-[[核酮糖5-磷酸]] + [[二氧化碳|CO<sub>2</sub>]] + NADPH

在上述反应中，6-[[磷酸葡萄糖酸]]首先被氧化为3-[[酮]]-6-磷酸葡萄糖酸，同时NADPH形成并被释放；然后，[[中间体]]被[[脱羧反应|脱羧]]产生1,2-烯二醇式结构，最终[[互变异构化]]为核酮糖5-磷酸。<ref>{{cite journal | author = Chen YY, Ko TP, Chen WH, Lo LP, Lin CH, Wang AH | year = 2010 | title = Conformational changes associated with cofactor/substrate binding of 6-phosphogluconate dehydrogenase from Escherichia coli and Klebsiella pneumoniae: Implications for enzyme mechanism | journal = J Struct Biol | volume = 169 | pages = 25–35 | pmid = 19686854 | issue = 1 | doi = 10.1016/j.jsb.2009.08.006 }}</ref> 高[[浓度]]的NADPH对这种酶的活性有抑制性，而6-磷酸葡萄糖酸则能激活这种酶。<ref>{{ cite journal | doi = 10.1016/S0167-4838(98)00222-2 | author = Rippa M, Giovannini PP, Barrett MP, Dallocchio F, Hanau S | year = 1998| title = 6-Phosphogluconate dehydrogenase: the mechanism of action investigated by a comparison of the enzyme from different species | journal = Biochim Biophys Acta | volume = 1429 | pages = 83–92 | pmid = 9920387 | issue = 1 }}</ref> 磷酸葡萄糖酸脱氢酶 (脱羧)主要参与核酮糖5-磷酸的产生，并为[[磷酸戊糖途径]]提供主要的NADPH。<ref>{{cite journal | author = He, W., Wang, Y., Liu, W. & Zhou, C.-Z| year = 2007| title = Crystal structure of Saccharomyces cerevisiae 6-phosphogluconate dehydrogenase Gnd1 | journal = BMC Struct. Biol | volume = 7, (38) | pmid = 17570834 | pages = 38 | doi = 10.1186/1472-6807-7-38 | pmc = 1919378 }}</ref>

== 参考文献 ==
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{{reflist}}
* {{cite journal | author = DICKENS F, GLOCK GE | year = 1951 | title = Direct oxidation of glucose-6-phosphate, 6-phosphogluconate and pentose-5-phosphates by enzymes of animal origin | url = https://archive.org/details/sim_biochemical-journal_1951-11_50_1/page/81 | journal = Biochem. J.  | volume = 50 | pages = 81–95  | pmid = 14904376 | issue = 1 | pmc = 1197610 }}
* {{cite journal | author = Bonsignore A and Horecker BL | year = 1961 | title = Purification and properties of beta-L-hydroxy acid dehydrogenase II. Isolation of beta-keto-L-gluconic acid, an intermediate in L-xylulose biosynthesis | url = https://archive.org/details/sim_journal-of-biological-chemistry_1961-11_236_11/page/2975 | journal = J. Biol. Chem.  | volume = 236 | pages = 2975–2980 }}
* {{cite journal | author = Scott DBM and Cohen SS | year = 1953 | title = The oxidative pathway of carbohydrate metabolism in ''Escherichia coli''. 1. The isolation and properties of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase | url = https://archive.org/details/sim_biochemical-journal_1953-08_55_1/page/23 | journal = Biochem. J.  | volume = 55 | pages = 23–33 }}
* {{cite journal | author = Scott DBM and Cohen SS | year = 1957 | title = The oxidative pathway of carbohydrate metabolism in ''Escherichia coli''. 5. Isolation and identification of ribulose phosphate produced from 6-phosphogluconate by the dehydrogenase of E. coli | url = https://archive.org/details/sim_biochemical-journal_1957-04_65_4/page/686 | journal = Biochem. J.  | volume = 65 | pages = 686–689 }}
{{refend}}

{{EC 1.1}}

[[Category:EC 1.1.1]]
[[Category:NADPH依赖性酶]]
[[Category:已知结构的酶]]
[[Category:锰酶]]