查看“︁异柠檬酸脱氢酶 (NADP+)”︁的源代码

{{DISPLAYTITLE:異檸檬酸脱氫酶 (NADP<sup>+</sup>)}}
{{enzyme
| Name = 異檸檬酸脱氫酶 (NADP<sup>+</sup>)
| Systematic_name = isocitrate:NADP<sup>+</sup> oxidoreductase (decarboxylating)
| Acronym = IDP; IDP1; IDP2; IDP3; IDH（有歧义）
| Other_names = oxalosuccinate decarboxylase; isocitrate dehydrogenase (NADP); oxalsuccinic decarboxylase; isocitrate (NADP) dehydrogenase; isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase; NADP-specific isocitrate dehydrogenase; NADP-linked isocitrate dehydrogenase; NADP-dependent isocitrate dehydrogenase; NADP isocitric dehydrogenase; isocitrate dehydrogenase (NADP-dependent); NADP-dependent isocitric dehydrogenase; triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase; NADP<sup>+</sup>-linked isocitrate dehydrogenase; dual-cofactor-specific isocitrate dehydrogenase; NADP<sup>+</sup>-ICDH; NADP<sup>+</sup>-IDH
| EC_number = 1.1.1.42
| CAS_number = 9028-48-2 | IUBMB_EC_number = 1/1/1/42 | GO_code = 0004450 | image = Isocitrate dehydrogenase of Escherichia coli Complex.jpg
| width = 
| caption = [[大肠杆菌]]（''E. coli''）異檸檬酸脱氫酶(NADP<sup>+</sup>)的晶体结构（[[蛋白质数据库|PDB]] 1CW7）<ref>{{cite journal | author = Cherbavaz DB, Lee ME, Stroud RM, Koshland DE | title = Active site water molecules revealed in the 2.1 A resolution structure of a site-directed mutant of isocitrate dehydrogenase | journal = J. Mol. Biol. | volume = 295 | issue = 3 | pages = 377–85 | pmid = 10623532 | doi = 10.1006/jmbi.1999.3195 | url = | issn = |date=January 2000}}</ref> 这种酶有三个活性位点，三[[分子]]柠檬酸中的其中一个在NADP<sup>+</sup>结合区域中。
}}
'''異檸檬酸脱氫酶 (NADP<sup>+</sup>)'''（{{lang-en|isocitrate dehydrogenase (NADP<sup>+</sup>)}}，[[EC编号|EC]] [http://enzyme.expasy.org/EC/1.1.1.42 1.1.1.42] {{Wayback|url=http://enzyme.expasy.org/EC/1.1.1.42 |date=20220420043440 }}）是一种以[[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]]或[[烟酰胺腺嘌呤二核苷酸磷酸|NADP<sup>+</sup>]]为[[受体 (生物化学)|受体]]、作用于[[供体]][[次甲基|CH]]-[[羟基|OH]][[基团]]上的[[氧化还原酶]]。这种[[酶]]能[[催化]]以下[[酶促反应]]：

:[[異檸檬酸]] + [[烟酰胺腺嘌呤二核苷酸磷酸|NADP<sup>+</sup>]] <math>\rightleftharpoons</math> [[2-酮戊二酸]] + [[二氧化碳|CO<sub>2</sub>]] + NADPH

異檸檬酸脱氫酶 (NADP<sup>+</sup>)需要[[二价锰离子|Mn<sup>2+</sup>]]或[[二价镁离子|Mg<sup>2+</sup>]]以维持活性。[[真核细胞]]中的异柠檬酸脱氢酶 (NAD<sup>+</sup>)以两种形式存在：其中一种与NADP<sup>+</sup>还存在于[[细胞质基质]]中不能变构调节；另一种与NAD<sup>+</sup>相关联且仅在[[线粒体]]中被发现并但可以变构调节。<ref>{{cite book | author = Camacho, M.L., Brown, R.A., Bonete, M.J., Danson, M.J. and Hough, D.W. | title = Isocitrate dehydrogenases from ''Haloferax volcanii'' and ''Sulfolobus solfataricus'': enzyme purification, characterisation and N-terminal sequence. | journal = FEMS Microbiol. Lett. | volume = 134 | year = 1995 | pages = 85-90 | PMID = 8593959 | url = http://www.ncbi.nlm.nih.gov/pubmed/8593959?dopt=Abstract | language = en | access-date = 2012-05-08 | archive-date = 2019-03-23 | archive-url = https://web.archive.org/web/20190323170714/https://www.ncbi.nlm.nih.gov/pubmed/8593959?dopt=Abstract }}</ref> 这两种酶——异柠檬酸脱氢酶 (NADP<sup>+</sup>)与[[异柠檬酸脱氢酶 (NAD+)|异柠檬酸脱氢酶 (NAD<sup>+</sup>)]]并不相同，前者能以[[草酰琥珀酸]]作为[[底物]]。某些[[物种]]的异柠檬酸脱氢酶 (NADP<sup>+</sup>)也能利用NAD<sup>+</sup>，但催化的[[反应速率]]较慢。<ref>{{cite journal | author = Steen, I.H., Lien, T. and Birkeland, N.-K. | title = Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, ‘’Archaeoglobus fulgidus’’. | journal = Arch. Microbiol. | volume = 168 | year = 1997 | pages = 412-420 | PMID = 9325430 | language = en}}</ref>

== 参考文献 ==
{{reflist}}
{{refbegin}}
*{{cite journal | author = Agosin, M.U. and Weinbach, E.C. | title = Partial purification and characterization of the isocitric dehydrogenase from ‘’Trypanosoma cruzi’’. | journal = Biochim. Biophys. Acta | volume = 21 | year = 1956 | pages = 117-126 | PMID = 13363868 | language = en}}
* {{cite journal | author = Moyle, J. and Dixon, M. | title = Purification of the isocitrate enzyme (triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase). | url = https://archive.org/details/sim_biochemical-journal_1956-08_63_4/page/548 | journal = Biochem. J. | volume = 63 | year = 1956 | pages = 548-552 | PMID = 13355848 | language = en}}
* {{cite book | author = Plaut, G.W.E. | chapter = Isocitrate dehydrogenases | editor = Lardy, H. and Myrbäck, K. | title = The Enzymes | edition = 2nd | volume = 7 | publisher = Academic Press | location = New York | year = 1963 | pages = 105-126 | language = en}}
* {{cite journal | author = Siebert, G., Dubuc, J., Warner, R.C. and Plaut, G.W.E. | title = The preparation of isocitrate dehydrogenase from mammalian heart. | journal = J. Biol. Chem.|volume = 226 | year = 1957 | pages = 965-975 | PMID = 13438885 | language = en}}
* {{cite book | author = Vickery, H.B. A | title = suggested new nomenclature for the isomers of isocitric acid. | journal = J. Biol. Chem. | volume = 237 | year = 1962 | pages = 1739-1741 | PMID = 13925783 | language = en}}
* {{cite book | author = Koh, H.J., Lee, S.M., Son, B.G., Lee, S.H., Ryoo, Z.Y., Chang, K.T., Park, J.W., Park, D.C., Song, B.J., Veech, R.L., Song, H. and Huh, T.L. | title = Cytosolic NADP<sup>+</sup>-dependent isocitrate dehydrogenase plays a key role in lipid metabolism. | journal = J. Biol. Chem. 279  | year = 2004 | pages = 39968-39974 | PMID = 15254034 | language = en}}
* {{cite journal | author = Ceccarelli, C., Grodsky, N.B., Ariyaratne, N., Colman, R.F. and Bahnson, B.J. | title = Crystal structure of porcine mitochondrial NADP<sup>+</sup>-dependent isocitrate dehydrogenase complexed with Mn<sup>2+</sup> and isocitrate. Insights into the enzyme mechanism. | journal = J. Biol. Chem. 277 | year = 2002 | pages = 43454-43462 | PMID = 12207025 | language = en}}
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{{EC 1.1}}

[[Category:EC 1.1.1]]
[[Category:NADPH依赖性酶]]
[[Category:已知结构的酶]]
{{DEFAULTSORT:異檸檬酸脱氫酶_(NADP+)}}