查看“︁异柠檬酸脱氢酶 (NAD+)”︁的源代码

{{DISPLAYTITLE:异柠檬酸脱氢酶 (NAD<sup>+</sup>)}}
{{enzyme
| Name = 异柠檬酸脱氢酶 (NAD<sup>+</sup>)
| Systematic_name = isocitrate:NAD<sup>+</sup> oxidoreductase (decarboxylating)
| Acronym = IDH（有歧义）
| Other_names = isocitric dehydrogenase; β-ketoglutaric-isocitric carboxylase; isocitric acid dehydrogenase; NAD dependent isocitrate dehydrogenase; NAD isocitrate dehydrogenase; NAD-linked isocitrate dehydrogenase; NAD-specific isocitrate dehydrogenase; NAD isocitric dehydrogenase; isocitrate dehydrogenase (NAD); nicotinamide adenine dinucleotide isocitrate dehydrogenase
| EC_number = 1.1.1.41
| CAS_number = 9001-58-5 | IUBMB_EC_number = 1/1/1/41 | GO_code = 0004449 | image = Isocitrate dehydrogenase.png
| width = 
| caption = 结合了[[柠檬酸]]（[[洋红色]]小[[分子]]）和[[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]]（[[白色]]）的异柠檬酸脱氢酶 (NAD+)<ref>{{cite journal | author = A. D. Mesecar, B. L. Stoddard, D. E. Koshland | title = Orbital steering in the catalytic power of enzymes: small structural changes with large catalytic consequences. | journal = Science | volume = 277 | pages = 202-206 | year = 1997}}</ref>（[[蛋白质数据库|PDB]] 3blv）
}}
'''异柠檬酸脱氢酶 (NAD<sup>+</sup>)'''（{{lang-en|isocitrate dehydrogenase (NAD+)}}，[[EC编号|EC]] [http://enzyme.expasy.org/EC/1.1.1.41 1.1.1.41] {{Wayback|url=http://enzyme.expasy.org/EC/1.1.1.41 |date=20220615163912 }}）是一种以[[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]]或[[烟酰胺腺嘌呤二核苷酸磷酸|NADP<sup>+</sup>]]为[[受体 (生物化学)|受体]]、作用于[[供体]][[次甲基|CH]]-[[羟基|OH]][[基团]]上的[[氧化还原酶]]。这种[[酶]]能[[催化]]以下[[酶促反应]]：

:[[异柠檬酸]] + [[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]] <math>\rightleftharpoons</math> [[2-酮戊二酸]] + [[二氧化碳|CO<sub>2</sub>]] + NADH

异柠檬酸脱氢酶 (NAD<sup>+</sup>)需要二价锰离子(Mn<sup>2+</sup>)或二价镁离子(Mg<sup>2+</sup>)以维持活性。[[真核细胞]]中的异柠檬酸脱氢酶 (NAD<sup>+</sup>)以两种形式存在：其中一种与NAD<sup>+</sup>相关联且仅在[[线粒体]]中被发现并可以[[变构调节]]；另一种与NADP<sup>+</sup>还存在于[[细胞质基质]]中但不能[[变构调节]]。<ref>{{cite book | author = Camacho, M.L., Brown, R.A., Bonete, M.J., Danson, M.J. and Hough, D.W. | title = Isocitrate dehydrogenases from ''Haloferax volcanii'' and ''Sulfolobus solfataricus'': enzyme purification, characterisation and N-terminal sequence. | journal = FEMS Microbiol. Lett. | volume = 134 | year = 1995 | pages = 85-90 | PMID = 8593959 | url = http://www.ncbi.nlm.nih.gov/pubmed/8593959?dopt=Abstract | language = en | access-date = 2012-05-08 | archive-date = 2019-03-23 | archive-url = https://web.archive.org/web/20190323170714/https://www.ncbi.nlm.nih.gov/pubmed/8593959?dopt=Abstract }}</ref> 这两种酶——异柠檬酸脱氢酶 (NAD<sup>+</sup>)与[[异柠檬酸脱氢酶 (NADP+)|异柠檬酸脱氢酶 (NADP<sup>+</sup>)]]并不相同，后者不能以[[草酰琥珀酸]]作为[[底物]]。某些[[物种]]的异柠檬酸脱氢酶 (NAD<sup>+</sup>)也能利用NADP<sup>+</sup>，但催化的[[反应速率]]较慢。<ref>{{cite journal | author = Inoue, H., Tamura, T., Ehara, N., Nishito, A., Nakayama, Y., Maekawa, M., Imada, K., Tanaka, H. and Inagaki, K. | title = Biochemical and molecular characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph ''Acidithiobacillus thiooxidans''. | journal = FEMS Microbiol. Lett. | volume = 214 | year = 2002 | pages = 127-132 | PMID = 12204383 | url = http://www.ncbi.nlm.nih.gov/pubmed/12204383?dopt=Abstract | language = en | access-date = 2012-05-08 | archive-date = 2019-03-23 | archive-url = https://web.archive.org/web/20190323052751/https://www.ncbi.nlm.nih.gov/pubmed/12204383?dopt=Abstract }}</ref>

== 参考文献 ==
{{reflist}}
{{refbegin}}
* {{cite journal | author = Hathaway, J.A. and Atkinson, D.E. | title = The effect of adenylic acid on yeast nicotinamide adenine dinucleotide isocitrate dehydrogenase, a possible metabolic control mechanism. | journal = J. Biol. Chem. | volume = 238 | year = 1963 | pages = 2875-2881 | PMID = 14063317 | url = http://www.ncbi.nlm.nih.gov/pubmed/14063317?dopt=Abstract | language = en | access-date = 2012-05-08 | archive-date = 2019-03-25 | archive-url = https://web.archive.org/web/20190325154930/https://www.ncbi.nlm.nih.gov/pubmed/14063317?dopt=Abstract }}
* {{cite journal | author = Kornberg, A. and Pricer, W.E. | title = Di- and triphosphopyridine nucleotide isocitric dehydrogenase in yeast. | journal = J. Biol. Chem. | volume = 189 | year = 1951 | pages = 123-136 | PMID = 14832224 | url = http://www.ncbi.nlm.nih.gov/pubmed/14832224?dopt=Abstract | language = en | access-date = 2012-05-08 | archive-date = 2019-03-22 | archive-url = https://web.archive.org/web/20190322141204/https://www.ncbi.nlm.nih.gov/pubmed/14832224?dopt=Abstract }}
* {{cite book | author = Plaut, G.W.E. | chapter = Isocitrate dehydrogenases | editor = Lardy, H. and Myrbäck, K. | title = The Enzymes | edition = 2nd | volume = 7 | publisher = Academic Press | location = New York | year = 1963 | pages = 105-126 | language = en}}
* {{cite journal | author = Plaut, G.W.E. and Sung, S.-C. Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues. | title = J. Biol. Chem. | volume = 207 | year = 1954 | pages = 305-314 | PMID = 13152105 | url = http://www.ncbi.nlm.nih.gov/pubmed/13152105?dopt=Abstract | language = en | journal =  | access-date = 2012-05-08 | archive-date = 2019-03-25 | archive-url = https://web.archive.org/web/20190325154936/https://www.ncbi.nlm.nih.gov/pubmed/13152105?dopt=Abstract }}
* {{cite book | author = Ramakrishnan, C.V. and Martin, S.M. | title = Isocitric dehydrogenase in ''Aspergillus niger''. | journal = Arch. Biochem. Biophys. | volume = 55 | year = 1955 | pages = 403-407 | language = en}}
* {{cite journal | author = Vickery, H.B. | title = A suggested new nomenclature for the isomers of isocitric acid. | journal = J. Biol. Chem. | volume = 237 | pages = 1739-1741 | PMID = 13925783 | url = http://www.ncbi.nlm.nih.gov/pubmed/13925783?dopt=Abstract | language = en | access-date = 2012-05-08 | archive-date = 2019-03-23 | archive-url = https://web.archive.org/web/20190323125727/https://www.ncbi.nlm.nih.gov/pubmed/13925783?dopt=Abstract }}
* {{cite journal | author = Kim, Y.O., Koh, H.J., Kim, S.H., Jo, S.H., Huh, J.W., Jeong, K.S., Lee, I.J., Song, B.J. and Huh, T.L. | title = Identification and functional characterization of a novel, tissue-specific NAD<sup>+</sup>-dependent isocitrate dehydrogenase β subunit isoform. | journal = J. Biol. Chem. | volume = 274 | year = 1999 | pages = 36866-36875 | PMID = 10601238 | url = http://www.ncbi.nlm.nih.gov/pubmed/10601238?dopt=Abstract | language = en | access-date = 2012-05-08 | archive-date = 2019-03-22 | archive-url = https://web.archive.org/web/20190322141257/https://www.ncbi.nlm.nih.gov/pubmed/10601238?dopt=Abstract }}
{{refend}}

{{EC 1.1}}

[[Category:EC 1.1.1]]
[[Category:NADH依赖性酶]]
[[Category:已知结构的酶]]