查看“︁二碘苯丙酮酸还原酶”︁的源代码

{{enzyme
| Name = 二碘苯丙酮酸还原酶
| Systematic_name = 3-(3,5-diiodo-4-hydroxyphenyl)lactate:NAD<sup>+</sup> 2-oxidoreductase
| Acronym = KAR
| Other_names = aromatic α-keto acid; 2-oxo acid reductase
| EC_number = 1.1.1.96
| CAS_number = 37250-31-0
| IUBMB_EC_number = 1/1/1/96
| GO_code = 0047860
| width = 
| caption = 
}}
'''二碘苯丙酮酸还原酶'''（{{lang-en|diiodophenylpyruvate reductase}}，[[EC编号|EC]] [http://enzyme.expasy.org/EC/1.1.1.96 1.1.1.96]{{Wayback|url=http://enzyme.expasy.org/EC/1.1.1.96 |date=20200918180911 }}），是一种以[[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]]或[[烟酰胺腺嘌呤二核苷酸磷酸|NADP<sup>+</sup>]]为[[受体 (生物化学)|受体]]、作用于[[供体]][[次甲基|CH]]-[[羟基|OH]][[基团]]上的[[氧化还原酶]]。这种[[酶]]能[[催化]]以下[[酶促反应]]：

:3-(3,5-二[[碘]]-4-羟苯基)[[乳酸]] + [[烟酰胺腺嘌呤二核苷酸|NAD<sup>+</sup>]] <math>\rightleftharpoons</math> 3-(3,5-二碘-4-羟苯基)[[丙酮酸]] + NADH + [[氢离子|H<sup>+</sup>]]

二碘苯丙酮酸还原酶[[分子]][[等电点]]为5.4，由两个相同的[[蛋白质亚基]]组成（亚基[[相对分子质量]]约为40,000[[原子质量单位|D]]），[[人类]]二碘苯丙酮酸还原酶亚基由[[12号染色体 (人类)|12号染色体]]上的[[基因]]编码，可能与[[乳酸脱氢酶|乳酸脱氢酶B]]有近缘关系。<ref>{{cite journal | author = L. J. Donald | title = A description of human aromatic α-keto acid reductase | journal = Annals of Human Genetics | volume = 46 | issue = 4 | pages = 299-306 | doi = 10.1111/j.1469-1809.1982.tb01581.x | url = http://onlinelibrary.wiley.com/doi/10.1111/j.1469-1809.1982.tb01581.x/abstract | date = Oct 1982 | access-date = 2012-08-03 | archive-date = 2017-04-10 | archive-url = https://web.archive.org/web/20170410074031/http://onlinelibrary.wiley.com/doi/10.1111/j.1469-1809.1982.tb01581.x/abstract | dead-url = no }}</ref>这种酶的[[底物]]还可以是其他含有以[[丙酮酸]]支链作为[[取代基]]的[[芳环]]，其中[[卤化物|卤代类]][[衍生物]]作为底物具有较高活性。底物的活性从大到小按顺序排列为：[[草酰乙酸]]、3,5-二碘-4-羟苯基丙酮酸、[[吲哚丙酮酸]]、[[苯丙酮酸]]，而[[乙醛酸]]、[[丙酮酸]]、[[羟基丙酮酸]]以及在芳环3位或5位上有[[羟基]]或[[氨基]]的底物则仅具有极低活性或不具活性。<ref>{{cite journal | author = Yoshikazu Takada, Tomoo Noguchi, Ryo Kido | title = Purification, characterization and identification of aromatic2-oxoacidreductase | journal = Life Sciences | volume = 20 | issue = 4 | pages = 609-616 | doi = 10.1016/0024-3205(77)90463-5 | url = http://www.sciencedirect.com/science/article/pii/0024320577904635 |date=Feb 15, 1977}}</ref>

== 参考文献 ==
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* {{cite journal | author = Zannoni VG, Weber WW | date = 1966 | title = Isolation and properties of aromatic alpha-keto acid reductase | journal = J. Biol. Chem.  | volume = 241 | pages = 1340-1344  | pmid = 5935348 | issue = 6 }}
{{refend}}

{{EC 1.1}}

[[Category:EC 1.1.1]]
[[Category:NADH依赖性酶]]
[[Category:未知结构的酶]]